WebDec 22, 2024 · Significantly, precise mutagenesis mediated by preassembled primer editor (PE) RNPs led to 50% GFP reporter gene recovery in protoplasts and up to 4.6% editing frequency for the specific AtPDS mutation in the genome. The rapid, versatile and efficient gene editing by CRISPR RNP variants in protoplasts provides a valuable platform for ... WebDelivering RNPs involves binding of the Cas enzyme to the gRNA on the bench—outside of the cell—and then bringing it into the cell as a complete RNP. If delivering an expression vector such as plasmid or virus instead of RNP, there is greater risk of off-target cuts and/or integration of plasmid or viral vector DNA (including the Cas gene itself) in the genome of …
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