Pronex® size-selective purification system
WebApr 1, 2024 · The library was then purified using the ProNex® Size Selective Purification System (Promega, WI, USA). After ProNex ® purification, the quantity of each sample was normalized to ensure equal representation of the library with the pooled samples using the ProNex ® NGS Library Quant Kit (Promega, WI, USA). WebThe ProNex® Size-Selective Purification System enables the rapid and efficient magnetic resin-based purification of double-stranded DNA (dsDNA) for next-generation sequencing …
Pronex® size-selective purification system
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WebThe libraries were purified with the ProNex Size-Selective Purification System (Promega, Madison, WI, USA). This was conducted by adding 220 µL of ProNex Chemistry (Promega, Madison, WI, USA) to 110 µL of each library, followed by two washing steps and the elution of the purified libraries. WebThe ProNex tm Size-Selective Purification System enables rapid and efficient magnetic resin-based purification of dsDNA for NGS, PCR and general molecular biology …
WebJul 14, 2024 · PCR products from RT-qPCR and qPCR were purified with ProNex Size Selective Purification System (Promega) following the manufacturer's protocol. Five nanograms of purified PCR product was used for Sanger sequencing with SeqStudio Genetic Analyzer (Thermo Fisher, Waltham, MA, USA). Results were visualised and analysed with … WebJun 26, 2024 · The purified and size selected DNA were first prepared for adapter ligation using the NEBNext Ultra II End Repair/dA Tailing Module and purified again using ProNex. The ligation of the Barcode Adapters (BCA) was done using the NEB Blunt/TA Ligase MasterMix for 15 min at 25°C.
WebThe ProNex® System allows users to select the desired size of purified dsDNA fragments, from 100bp to 750bp. The novel reagent formulation provides significantly improved … WebProNex® Size-Selective Purification System Magnetic resin-based purification of dsDNA for NGS, PCR and general molecular biology applications. NG2001, NG2002, NG2003 Let's find the product that meets your needs. Talk to a Scientist Manos UK
WebProNex® Size-Selective Purification System (Cat.# NG2001) MagneSphere® Technology Magnetic Separation Stand (Cat.# Z5332) ... Percentage of total reads categorized by size grouping and purification method. Graph includes only reads 50kb or greater for each sample. The percentage of reads for each 10kb size grouping is displayed using
WebThe ProNex® System allows users to select the desired size of purified dsDNA fragments, from 100bp to 750bp. The novel reagent formulation provides significantly improved selectivity, reproducibility and yield relative to traditional dsDNA purification methods. ibm full stack cloud developer jobWebOct 31, 2024 · ProNex Size-Selective Purification System (Promega), including bead solution, washing buffer, and elution buffer. 5. An automated electrophoresis tool for DNA and RNA sample quality control. 6. A benchtop fluorometer to accurately measure DNA and RNA. 2.4 PCR Amplification 1. A ready-to-use qPCR ibm fusion storageWebMabSelect SuRe LX is a protein A affinity resin with very high dynamic binding capacity at extended residence times. The chromatography resin is developed for processing of … ibm furloughWebProNex® Size-Selective DNA Purification System 10ml ¥ 50,000 効率的で精確なサイズ選択 ProNex® Size-Selective Purification Systemは、NGS、PCRおよび一般的な分子生物学ア … ibm full stack cloud developer professionalWebPromega ProNex Size-Selective Purification System, 10ml. The ProNex Size-Selective Purification System enables the rapid and efficient magnetic resin-based purification of … monatsticket bayern dbWebJun 3, 2024 · First-strand cDNA was synthesized with 6 cycles and size-selected using ProNex Size-Selective Purification System (Promega). Libraries were PCR amplified for 8–13 cycles and sequencing was performed on a Illumina HiSeq1500 sequencer. Library strategy: RIP-Seq: Library source: transcriptomic: Library selection: ibm fun factsWebPromega ProNex Size-Selective Purification System, 125ml. The ProNex Size-Selective Purification System enables the rapid and efficient magnetic resin-based purification of … ibm full stack cloud developer certificate