Isg54 minimal promoter
WebMay 5, 2024 · RAW-Lucia cells stably expressed an interferon sensitive response element from the mouse Isg54 minimal promoter and five interferon-stimulated response … We would like to show you a description here but the site won’t allow us. WebTo cite this cell line use: RAW-Lucia ISG (RRID:CVCL_X596) Comments. Characteristics: The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). Transfected with: Lucia luciferase, a synthetic gene designed on natural secreted luciferase genes from marine ...
Isg54 minimal promoter
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WebEventually, the effector cells release cytotoxic particles that kill the target cells. Jurkat NFAT-CD16 cells have been designed as effector reporter cells for our ADCC analysis. These cells stably express the Lucia luciferase reporter gene under the control of ISG54 minimal promoter fused with six NFAT response elements. Web74 an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. 75 Detectable levels of secreted luciferase as a read out for IFN activation was observed in a 76 temporal manner in response to Mtb Erdman at a MOI of 5 with 10-15 folds activity by 24h and 77 48h (Fig. 1A).
WebDear researcher, minimal promoter refers to a minimal sequence of a native promoter (mostly core promoter) that could express a downstream gene. WebOct 11, 2024 · To confirm the effect observed at the transcriptional level, we assessed the production of proteins dependent on the ISRE promoter. Indeed, A549 Dual are reporter cells Lucia luciferase under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements (ISRE). Hence, we assessed the release of secreted ...
Weband c-GAMP), but not other CDNs (e.g. c-diGMP), bind to the G-protein-coupled surface receptor A2a in monocytes. Thus, it is currently not known whether other receptors for surface binding and/or cellular uptake o f CDNs exist. Delivery o f CDNs into cells For practical applications that involve addition of CDNs directly to cell cultures, blood o r … WebJun 30, 2024 · Interferon (IFN)-stimulated gene (ISG) 56 family (composed of ISG54, ISG56, ISG58, and ISG60) plays important roles in defense against viral infection in mammalian …
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WebHere we examined the role of IRF3 in expression of innate anti-viral proteins produced in response to IFN-γ plus TLR3 agonist. Wild-type (WT) and IRF3KO RAW264.7 cells, each with ISG54-promoter-luciferase reporter vectors, were stimulated with IFN-γ, poly I:C, or both together. ISG54 promoter activity was significantly reduced in IRF3KO ... proxy fetchWebApr 3, 2014 · In practice, the term "promoter" describes the combination of the promoter (RNA polymerase binding site) and operators (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target genes. The sequence of the promoter region controls the binding of the RNA polymerase and transcription factors, … proxy fhwsWebgene, that encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements, and (II) a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an IFN-β minimal promoter fused to 5 copies of the NF-κB consensus proxy-fehlerWebNov 25, 2003 · It was previously reported that histone H3 becomes hyperacetylated in ISGF3 target promoters in response to IFN-α, so the effects of IFN-α on histone H4 acetylation … proxy fernuni hagenWebMay 12, 2009 · (A) ISG56 and ISG54 inhibit SeV-induced activation of the IFN-β promoter in a dose-dependent manner. 293 cells (1 × 10 5) were transfected with an IFN-β promoter reporter (0.1 μg) and increased amount of ISG56 or ISG54 expression plasmids. Eighteen hours after transfection, cells were left uninfected or infected with SeV for 12 h before ... proxy featuresrestoration hardware cartwright bath cabinetWebA549-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites. A549-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN ... proxy fees